Cell Lines

FARM cell lines are available free of charge. Our inventory currently includes human and mouse fibroblasts along with the FA Cancer Cell Line Resource.

FA Cancer Cell Line Resource

  • Sporadic HNSCC derived, FANC-isogenic cell line trios

  • COMING SOON! Fanconi patient-derived head and neck squamous cell carcinoma (HNSCC) FANC-isogenic cell line pairs 5 independent FA patient-derived isogenic HNSCC cell line pairs from FANCA, FANCC and FANCL complementation groups.

Storage, Plating, and Media

How should I store my cells?
Cells should be stored in liquid nitrogen or thawed immediately upon arrival.

How should I plate my cells?
Cells are frozen in 10% DMSO. You can dilute the DMSO down to 0.5%, or remove it by washing/spinning. Please make sure to check media requirments listed on a cell's datasheet or below (continue reading!). Plating cells on a 10 cm plate is usually recommended. However, if you find the cell pellet is significantly larger or smaller adjust the plate size accordingly.

What media should I use? 

Human and Mouse Fibroblasts

Immortalized primary FA human fibroblasts: Alpha-MEM supplemented with 10-16% fetal bovine serum and 1% Pen/Strep. See datasheet for details and selection media

Immortalized mouse FA knockout and wildtype fibroblasts:  High glucose DMEM supplemented with 10% FBS and 1% Pen/Strep. Add L-glutamine if it is missing from the DMEM. 

FA Cancer Cell Line Resource - Isogenic head and neck squamous cell carcinoma (HNSCC) cell line trios:

JHU-SCC-FaDu: Eagle’s Minimum Essential Media (Corning 10-010-CV), 10% fetal bovine serum (FBS, Hyclone Laboratories, SH30071.03), and 1% penicillin-streptomycin (Gibco #10378016). See datasheet for details.

  • JHU-SCC-FaDuko+Tg selection media: 100 ug/ml hygromycin B. Antibiotic selection of JHU-SCC-FaDuko+Tg cells after thawing and prior to experiments is recommended.

UM-SCC-01: Dulbecco’s Modified Eagle Medium – High glucose (Sigma D5796), 10% fetal bovine serum (FBS, Hyclone Laboratories SH30071.03), 1% penicillin-streptomycin (Gibco 10378016), and 1% non-essential amino acids (Gibco 11140050). See datasheet for details. 

  • UM-SCC-01ko+Tg selection media: 100 ug/ml hygromycin B. Antibiotic selection of UM-SCC-01ko+Tg cells after thawing and prior to experiments is recommended.

SFCI-SCC-09: DMEM/F-12 (Gibco 11320033), 0.5 mM sodium pyruvate (Gibco 11360070), 400 ng/mL hydrocortisone (Stemcell Technologies 74144), 10% fetal bovine serum (FBS, Hyclone Laboratories SH30071.03), and 1% penicillin-streptomycin (Gibco 10378016). See datasheet for details.

  • SFCI-SCC-09ko+Tg selection media: 100 ug/ml hygromycin B. Antibiotic selection of SFCI-SCC-09ko+Tg cells after thawing and prior to experiments is recommended.
  • It was observed that these cells grow much slower at low density, so maintaining at least 1.0x10e6 cells in each plate (10cm) at a time will help as you expand. See datasheet for details. 

CAL-SCC-27: Dulbecco's Modified Eagle's Medium (ATCC 30­-2002), 10% fetal bovine serum, and 1% Pen/Strep. See datasheet for details. 

CAL-SCC-33: 90% Dulbecco's MEM,  10% h.i. FBS, and 2 mM L-glutamine. See datasheet for details. 

Immortalized primary FA human fibroblasts

Name Cell Line Datasheet Order
FANCA PD220 PD220.pdf
FANCC PD331 PD331.pdf
FANCD2 PD20 PD20 & PD20RVD2.pdf
FANCD2 Tg+ Complemented PD20 RV:D2 PD20 & PD20RVD2.pdf
FANCG PD352 PD352 .pdf

Immortalized mouse FA knockout and wildtype fibroblasts

Name Cell Line Datasheet Order
Fanc (+/+) 2408 (wildtype control)
Fanca (-/-) 2403
Fancc (-/-) 2421
Fanca(-/-) Fancc(-/-) 2422
Fancd2(+/+) Aldh2 (+/+) 5029 (wildtype control)
Fancd2(-/-) 5017
Fancd2(-/-) Aldh2(-/-) 5035

Sporadic HNSCC derived, FANC-isogenic cell line trios

Name Cell Line Datasheet Order
CAL-SCC-27 trio parental, FANCA knockout, FANCA retroviral Tg+ CAL27+clones.pdf
CAL-SCC-33 trio parental, FANCA knockout, FANCA retroviral Tg+ CAL33+clones.pdf
JHU-SCC-FaDu trio parental, FANCA knockout, FANCA safe harbor Tg+ JHU-SCC-FaDu.pdf
UM-SCC-01 trio parental, FANCA knockout, FANCA safe harbor Tg+ UM-SCC-01.pdf
SFCI-SCC-09 trio parental, FANCA knockout, FANCA safe harbor Tg+ SFCI-SCC-09.pdf