Frequently Asked Questions

Using Materials

What is the species for the antibodies?

All of our antibodies are affinity purified rabbit polyclonal.

How should I store the antibodies?

Antibodies stored in PBS should be kept at 4°C.

Antibodies stored in 50% Glycerol, .05% Azide, and 1% BSA can be safely stored at either 4°C, or at -20°C for longer term storage.

Please refer to the data sheets listed on the Antibodies page to determine optimal storage conditions for each antibody.

What dilution should I use for my antibodies?

We do not test any of the antibodies ourselves and rely on user feedback to determine the best applications and working conditions. Please refer to the data sheets linked on the Antibodies page to view user feedback. All investigators are required to submit use-data. When you are ready to submit use-data, refer to the Data Reporting Template to help guide your submission and contact Leslie Wakefield.

How should I store my cells?

Cells should be stored in liquid nitrogen or thawed immediately upon arrival.

How should I plate my cells?

Starting with a 10 cm plate is usually recommended. However, if you find that the cell pellet is significantly larger or smaller than what is typically plated on a 10 cm plate, adjust the plate size accordingly.

What media should I use?

For Mouse Embryonic Fibroblast cell lines, use high glucose DMEM supplemented with 10% FBS and 1% Pen/Strep.

For immortalized human fibroblast cell lines, use Alpha-MEM supplemented with 10% FBS and 1% Pen/Strep.

For head and neck squamous cell carcinoma (HNSCC) cell lines, use ATCC-formulated Eagle’s Minimum Essential medium (catalog #30-2003) with 10% FBS and 1% Pen/Strep.

Obtaining Materials

What is the cost for cells and antibodies?

All materials are free to academic institutions. You are only responsible for shipping costs.

I don't have a FedEx or UPS account number. What should I do?

You can contact FedEx or UPS to set up an account.

I am outside of the US. Can I still request materials from the repository?

Yes! However, it is YOUR responsibility to determine if any additional documents need to be included with the shipment to avoid delays at your local customs office. Contact your customs office and find out exactly what paperwork you'll need to import the materials. For some countries, like Australia or India, shipping options such as World Courier may be used to ensure that package(s) are maintained at the appropriate conditions during the long transit.