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OHSU # 2330 — Fluorescence microscope for high resolution imaging and single-molecule tracking

Summary

Despite improvements in the field of fluorescence microscopy, a major drawback of existing microscopes is their spectral resolution, as they can distinguish only up to 3-4 colors at a time and requiring multiple excitation sources, limiting their practical use in biomedical research.  Scientists at OHSU have recently solved this problem by developing the next-generation super resolution microscope (SRM) that allows high precision imaging of biological samples with 10-20 colors at once.  This advanced set up is fully compatible with live- or fixed-cell imaging, enables single-molecule tracking, and shows a 5-10 fold improvement in resolution over existing SRM techniques, thereby promising to be a transformative tool for studying complex, molecular interactions in intact cells.

 

Technology Overview 

An important caveat of SRM is color cross-talk.  Multiple colors within a single channel often compromises research experiments as it greatly limits the maximum number of fluorophores that can be resolved at a time, thereby slowing the rate of data acquisition.  Thus, there is an unmet need to develop multicolor imaging techniques that specifically address fluorescence bleed-through artifacts.

OHSU investigators have developed the Multispectral Super Resolution Microscopy (MSSRM) system - a powerful and versatile tool that enables simultaneous imaging of biological samples in 10-20 colors.  In contrast to the resolution of existing SRMs that is typically limited to 50-100 nm, MSSRM achieves a spectral resolution of 10 nm that allows it to distinguish multiple colors at once with essentially no cross-talk, using a single light source and without compromising spatial resolution.  This technique can be used to image both fixed (Fig 1) and live (Fig 2) cells.  The unique properties of this system allow many sub-cellular structures to be visualized in one experiment with high resolution.  In addition, this system enables multicolor single-molecule tracking in live cells in just one experiment.  This technology is compatible with most commercial, inverted microscopes commonly used for high resolution biological imaging.

 

Publication

Huang T, Phelps C, Wang J, Lin LJ, Bittel A, Scott Z, Jacques S, Gibbs SL, Gray JW, Nan X.  Simultaneous multicolor single-molecule tracking with single-laser excitation via spectral imaging.  Biophys J, 114(2) 301-310, 2018

 

Intellectual Property

US and Europe National stage applications

 

Licensing Opportunity

This technology is available for exclusive licensing.

 

Inventors:

Patents

Filed United States
Published United States WO 2018/085531
Published United States US-2019-0271592

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For more information, contact:

Anne Carlson
Technology Development Manager
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