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OHSU # 2639-A — Monoclonal antibody (7B1) against the α subunit of human epithelial sodium channel (hENaC)

Technology Overview
Monoclonal antibody (7B1) against the α subunit of the human epithelial sodium channel (hENaC). The antibody recognizes the tertiary, 3D epitope of the alpha subunit and is characterized as IgG2 with a kappa light chain. It binds with high affinity and specificity to the respective subunits constituting hENaC.

Epithelial sodium channels (ENaC) are involved in regulating Na+ homeostasis. ENaCs primarily require three different subunits to form a functional channel, α, β and γ.

Potential uses include:

  • purification of human ENaC for structure studies with an ability to purify and study ENaC from recombinant or native source.
  • determination of cellular location of the heterotrimeric ENaC by immunohistochemistry or immunocytochemistry.
  • differentiate ENaC formed from αβγ or δβγ subunits.
  • live detection of hENaC subunits in vivo following immunofluorescence labeling.
  • application for immunoprecipitation of intact heteromeric ENaC.
  • identification of human ENaC protein-expressing cells.

Publication
Noreng S et al., “Structure of the human epithelial sodium channel by cryo-electron microscopy.” Elife. 2018;7:e39340. Link

Licensing Opportunity
This technology is available for licensing.

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