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OHSU # 2049 — Sensor for Akt kinase activity in living cells
Technology Overview
Researchers at Oregon Health & Science University have developed a novel Akt biosensor which provides a robust readout for the PI3-kinase signaling pathway in living cells without the use complex microscopy techniques. This biosensor allows quantitative tracking of Akt activity for days in individual cells within a population, and responds within minutes to changes in the signaling pathway that is altered in a variety of disease states and physiological conditions. The biosensor provides a greater dynamic range, is more sensitive and more specific to changes in Akt activity than other FRET reporters, and does not require multiple fluorescent channels thus allowing for a second fluorescent reporter to be used for orthogonal monitoring of biochemical activity.
Publication
Gross SM, Rotwein P. Akt signaling dynamics in individual cells. J Cell Sci. 2015 Jul 15;128(14):2509-19. Link
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